| No. | Player | Pos | Ht | Wt | Class | Hometown | High School | Previous School |
|---|---|---|---|---|---|---|---|---|
| 1 | Will Fidler | QB | 6-4 | 229 lbs. | Jr | Henderson, KY | Henderson County | — |
| 2 | Qua Huzzie | LB | 5-10 | 210 lbs. | Fr | LaGrange, GA | LaGrange | — |
| 3 | Matt Roark | WR | 6-5 | 209 lbs. | So | Acworth, GA | North Cobb | — |
| 4 | Micah Johnson | LB | 6-2 | 258 lbs. | Sr | Ft. Campbell, KY | Ft. Campbell | — |
| 5 | Mike Hartline | QB | 6-6 | 206 lbs. | Jr | Canton, OH | GlenOak | — |
| 6 | Taiedo Smith | FS | 6-0 | 185 lbs. | So | Dunnellon, FL | Dunnellon | — |
| 7 | Ryan Mossakowski | QB | 6-4 | 208 lbs. | Fr | Frisco, TX | Centennial | — |
| 8 | Chris Matthews | WR | 6-5 | 210 lbs. | Jr | Los Angeles, CA | Dorsey | Los Angeles Harbor College |
| 9 | Ryan Tydlacka | K/P | 6-1 | 202 lbs. | So | Louisville, KY | Trinity | — |
| 10 | Matt Lentz | FS | 6-3 | 218 lbs. | So | Simpsonville, SC | Greenville | — |
| 11 | Greg Wilson | SS | 6-1 | 209 lbs. | So | College Park, GA | North Clayton | — |
| 12 | Morgan Newton | QB | 6-4 | 217 lbs. | Fr | Carmel, IN | Carmel | — |
| 13 | Eric Adeyemi | WR | 6-0 | 170 lbs. | So | Miami, FL | Southridge | — |
| 14 | Anthony Mosley | CB | 6-0 | 170 lbs. | So | Ellenwood, GA | Tucker | — |
| 14 | Ryan Phillippi | QB | 5-11 | 180 lbs. | Fr | Lexington, KY | Henry Clay | — |
| 15 | Martavious Neloms | CB | 6-1 | 180 lbs. | Fr | Memphis, TN | Fairley | — |
| 15 | Tyler Sargeant | QB | 6-4 | 208 lbs. | So | Waynesville, OH | Clinton Massie | — |
| 16 | La’Rod King | WR | 6-4 | 190 lbs. | Fr | Radcliff, KY | North Hardin | — |
| 17 | Jarvis Walker | S | 6-1 | 206 lbs. | Fr | Marrero, LA | Archbishop Rummel | — |
| 18 | Randall Cobb | QB/WR | 5-11 | 188 lbs. | So | Alcoa, TN | Alcoa | — |
| 19 | EJ Fields | WR | 6-1 | 198 lbs. | Fr | Frankfort, KY | Frankfort | — |
| 20 | Derrick Locke | RB | 5-9 | 190 lbs. | Jr | Hugo, OK | Hugo | — |
| 21 | Winston Guy | FS | 6-1 | 208 lbs. | So | Lexington, KY | Catholic | — |
| 22 | Danny Trevathan | LB | 6-1 | 220 lbs. | So | Leesburg, FL | Leesburg | — |
| 23 | Donald Russell | RB | 5-11 | 205 lbs. | Fr | West Palm Beach, FL | Dwyer | — |
| 24 | Randall Burden | CB | 6-0 | 170 lbs. | So | LaGrange, GA | LaGrange | — |
| 25 | Jonathan George | RB | 5-10 | 196 lbs. | Fr | Lincoln, AL | Lincoln | — |
| 26 | CoShik Williams | RB | 5-9 | 180 lbs. | Fr | Hiram, GA | Hiram | — |
| 26 | Rashaad Palmer | CB | 6-0 | 185 lbs. | Fr | Covington, KY | Holmes | — |
| 27 | Aaron Boyd | WR | 6-4 | 210 lbs. | So | Lexington, KY | Henry Clay | — |
| 27 | Ashton Cobb | SS | 6-0 | 215 lbs. | Sr | Aliquippa, PA | Center | — |
| 28 | Dakotah Tyler | RB | 5-11 | 205 lbs. | Fr | Indianapolis, IN | Pike | — |
| 29 | Alfonso Smith | RB | 6-1 | 208 lbs. | Sr | Louisville, KY | Waggener | — |
| 30 | Moncell Allen | RB | 5-7 | 225 lbs. | Jr | New Orleans, LA | Providence Day | Fork Union Military Academy |
| 30 | Zach Severance | CB | 5-11 | 188 lbs. | Fr | Reynoldsburg, OH | Reynoldsburg | Naval Acad. Prep School |
| 31 | Michael Harper | WR | 6-1 | 181 lbs. | Jr | Stone Mountain, GA | Stephenson | Howard |
| 31 | Mikie Benton | CB | 5-11 | 182 lbs. | Fr | Russellville, KY | Russellville | — |
| 32 | Trevard Lindley | CB | 6-0 | 179 lbs. | Sr | Hiram, GA | Hiram | — |
| 33 | Calvin Harrison | SS | 6-1 | 211 lbs. | Sr | Columbia, SC | Richland Northeast | — |
| 34 | Paul Warford | CB | 5-10 | 200 lbs. | Jr | Richmond, KY | Madison Central | — |
| 35 | Cartier Rice | CB | 5-10 | 185 lbs. | Fr | Duncan, SC | Byrnes | — |
| 36 | Lones Seiber | K | 5-9 | 190 lbs. | Sr | San Antonio, TX | Knoxville Central | — |
| 37 | Trey Bowland III | RB | 5-10 | 190 lbs. | Jr | Knoxville, TN | Christian Academy | — |
| 38 | John Conner | FB | 5-11 | 240 lbs. | Sr | West Chester, OH | Lakota West | — |
| 39 | William Johnson | LB | 6-2 | 205 lbs. | Fr | Nashville, TN | Pearl-Cohn | — |
| 40 | Daryl Faulkner | CB | 5-9 | 181 lbs. | Jr | Memphis, TN | Whitehaven | — |
| 40 | Maurice Grinter | TE | 6-3 | 253 lbs. | Sr | Louisville, KY | Fairdale | — |
| 41 | Brian Murphy | FB | 6-0 | 216 lbs. | So | Big Stone Gap, VA | Powell Valley | — |
| 42 | Nick Melillo | WR | 6-2 | 225 lbs. | So | Louisville, KY | Trinity | Lindenwood |
| 43 | Mikhail Mabry | LB | 6-2 | 248 lbs. | Sr | Mililani, HI | Mililani | — |
| 43 | Stephen Ball | FB | 5-10 | 229 lbs. | Jr | Brentwood, TN | Franklin Road | — |
| 44 | Clay Cecil | SS | 6-0 | 190 lbs. | Fr | Fort Thomas, KY | Highlands | — |
| 44 | Ryan Mosby | LB | 5-11 | 200 lbs. | Fr | Heath, TX | Rockwall-Heath | — |
| 45 | Antwane Glenn | DT | 6-3 | 273 lbs. | So | Pacolet, SC | Broome | — |
| 45 | Tatum Lewis | FB | 6-0 | 232 lbs. | Fr | Winchester, KY | George Rogers Clark | — |
| 46 | Ronnie Sneed | LB | 6-2 | 230 lbs. | So | Tallahassee, FL | Florida | — |
| 47 | A.J. Nance | FB | 5-11 | 256 lbs. | Sr | Knoxville, TN | Central | — |
| 48 | Alex Dutton | WR | 5-10 | 183 lbs. | Fr | Louisville, KY | Trinity | — |
| 48 | Ridge Wilson | LB | 6-3 | 207 lbs. | Fr | Louisville, KY | Central | — |
| 49 | Antonio Thomas | LB | 6-1 | 228 lbs. | So | Cowpens, SC | Broome | — |
| 49 | Nathan Dutton | WR | 5-10 | 185 lbs. | Fr | Louisville, KY | Trinity | — |
| 50 | Sam Maxwell | LB | 6-3 | 248 lbs. | Sr | Hartwell, GA | Hart County | — |
| 51 | Tristian Johnson | LB | 6-1 | 230 lbs. | Fr | LaGrange, GA | LaGrange | — |
| 52 | Billy Joe Murphy | OT | 6-6 | 298 lbs. | So | Gamaliel, KY | Monroe County | — |
| 53 | Ricky Lumpkin | DT | 6-4 | 294 lbs. | Jr | Clarksville, TN | Kenwood | — |
| 54 | Brandon Thurmond | LB | 6-2 | 226 lbs. | Jr | Rex, GA | Grady | — |
| 55 | DeQuin Evans | DE | 6-3 | 260 lbs. | Jr | Long Beach, CA | Cabrillo | Los Angeles Harbor College |
| 56 | Sam Simpson | OL | 6-4 | 280 lbs. | Fr | Lexington, KY | Henry Clay | — |
| 57 | Jacob Dufrene | LB | 6-1 | 220 lbs. | Jr | Cut Off, LA | John Curtis Christian | — |
| 58 | Sean Stackhouse | OG | 6-4 | 266 lbs. | Fr | Jacksonville, FL | Mandarin | — |
| 59 | Dave Ulinski | OG | 6-5 | 305 lbs. | Fr | Louisville, KY | duPont Manual | — |
| 59 | Jon Thomas | LS | 5-10 | 225 lbs. | So | Louisville, KY | St. Xavier | — |
| 60 | Dustin Luck | OG | 6-3 | 290 lbs. | Sr | Poole, KY | Henderson County | — |
| 61 | Jorge Gonzalez | C | 6-3 | 300 lbs. | Sr | Tampa, FL | Catholic | — |
| 62 | Greg Meisner | DE | 6-1 | 238 lbs. | So | Greensburg, PA | Hempfield Area | — |
| 63 | Jake Lanefski | OG/C | 6-4 | 286 lbs. | So | Mobile, AL | McGill-Toolen Catholic | — |
| 64 | Osaze Idumwonyi | C | 6-1 | 291 lbs. | Fr | Cedar Hill, TX | Cedar Hill | — |
| 65 | J.J. Helton | LS | 6-3 | 226 lbs. | Jr | Franklin, TN | Franklin | — |
| 66 | Chandler Burden | DE | 6-4 | 296 lbs. | So | Blue Ash, OH | LaSalle | — |
| 67 | Larry Warford | OL | 6-3 | 349 lbs. | Fr | Richmond, KY | Madison Central | — |
| 68 | Luke McDermott | DT | 6-1 | 260 lbs. | So | Louisville, KY | Trinity | — |
| 69 | Matt Smith | C | 6-4 | 280 lbs. | Fr | Louisville, KY | St. Xavier | — |
| 70 | Stuart Hines | OG | 6-4 | 295 lbs. | So | Bowling Green, KY | Bowling Green | — |
| 72 | Zipp Duncan | OT | 6-5 | 291 lbs. | Sr | Magnolia, KY | Elizabethtown | — |
| 73 | Donte Rumph | DL | 6-2 | 265 lbs. | Fr | St. Matthews, SC | Calhoun Co. | Fork Union Military Acad. |
| 74 | Trevino Woods | OT | 6-5 | 274 lbs. | Fr | Athens, GA | Clarke Central | — |
| 75 | Brad Durham | OT | 6-4 | 310 lbs. | Jr | Mount Vernon, KY | Rockcastle County | — |
| 76 | Justin Jeffries | OT | 6-6 | 320 lbs. | Sr | Louisville, KY | St. Xavier | — |
| 77 | Marcus Davis | C | 6-1 | 287 lbs. | Jr | Union, KY | Boone County | — |
| 78 | Christian Johnson | OG | 6-4 | 330 lbs. | Sr | Ft. Campbell, KY | West Potomac | Hargrave Military Academy |
| 78 | Jacob Lewellen | DE | 6-3 | 240 lbs. | Fr | Louisville, KY | Manual | — |
| 79 | Kevin Mitchell | OL | 6-6 | 295 lbs. | Fr | Winston, GA | Alexander | — |
| 80 | T.C. Drake | TE | 6-6 | 242 lbs. | Sr | Bardstown, KY | Nelson County | Hargrave Military Academy |
| 81 | Kyrus Lanxter | WR | 6-2 | 205 lbs. | Jr | Alcoa, TN | Alcoa | — |
| 82 | Anthony Kendrick | WR/TE | 6-3 | 220 lbs. | Fr | Katy, TX | Seven Lakes | — |
| 83 | Jordan Aumiller | ATH | 6-4 | 201 lbs. | Fr | Danville, KY | Boyle County | — |
| 84 | Charles Mustafaa | DT | 6-3 | 250 lbs. | So | College Park, GA | Westlake | — |
| 84 | Gabe Correll | TE | 6-3 | 222 lbs. | Fr | Cincinnati, OH | Anderson | Fork Union Military Academy |
| 85 | Gene McCaskill | WR | 6-0 | 182 lbs. | So | Chester, SC | Chester | — |
| 86 | Ross Bogue | TE | 6-5 | 244 lbs. | Sr | Suwanee, GA | North Gwinnett | — |
| 87 | Brian Adams | WR | 6-4 | 212 lbs. | Fr | Gainesville, GA | South Forsyth | — |
| 88 | Nik Brazley | WR | 5-9 | 170 lbs. | So | Louisville, KY | Male | — |
| 89 | Anthony Cecil | WR | 6-0 | 194 lbs. | Sr | Hodgenville, KY | LaRue County | — |
| 90 | Michael Gardner | DE | 6-4 | 208 lbs. | Fr | Lexington, KY | Henry Clay | — |
| 90 | Pat Simmons | K | 6-2 | 195 lbs. | Fr | LaGrange, GA | LaGrange | — |
| 91 | Corey Peters | DT | 6-3 | 295 lbs. | Sr | Louisville, KY | Central | — |
| 92 | Shane McCord | DT | 6-2 | 282 lbs. | Jr | Hartwell, GA | Hart County | — |
| 93 | Myron Walker Jr. | DL | 5-11 | 272 lbs. | Fr | Marrero, LA | Archbishop Rummel | — |
| 94 | Taylor Wyndham | DE | 6-4 | 230 lbs. | Fr | Swansea, SC | Swansea | — |
| 95 | Patrick Ligon | DE | 6-4 | 250 lbs. | Fr | Germantown, TN | Christian Brothers | — |
| 96 | Collins Ukwu | DE | 6-5 | 251 lbs. | Fr | La Vergne, TN | La Vergne | — |
| 97 | Mister Cobble | DT | 6-0 | 290 lbs. | Fr | Louisville, KY | Central | — |
| 98 | Mark Crawford | DT | 6-1 | 297 lbs. | So | Indianapolis, IN | Ben Davis | Coffeyville Community Coll. |
| — | Demetri Merritt | DE | 6-3 | 200 lbs. | Fr | LaGrange, GA | LaGrange | — |
| — | Justin Bean | WR | 6-2 | 180 lbs. | Fr | Tupelo, MS | Tupelo | — |
| — | Terrell Mitchell | TE | 6-6 | 220 lbs. | Fr | Hiram, GA | McEachern | — |
Source: University of Kentucky 2009 Football Media Guide. Heights, weights, classes and positions are season specific. A dash indicates an unassigned or unverified jersey number.